Green synthesis of nanoparticles provides an advancement over chemical and physical methods given its cost-effectively, environment-friendly, facile, and suitability for large-scale industrial production. Due to their characteristics, silver nanoparticles are exploited in various fields such as developing electronic devices, biosensing platforms, catalysts, wastewater purification, biomedicine, antifungal, and antibacterial agent. In this study, silver nanoparticles (Ag-NPs) were synthesized using leaves extract of Biarum chaduchrum at ambient temperature, and characterized through transmission electron microscopy, particle size analyzer, and fourier transform infrared spectroscopy. The average size of synthesized Ag-NPs was estimated at 100 nm with a spherical shape. In addition, the antibacterial activity of nanoparticles against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa was tested by minimum inhibitory concentration analysis. According to the results and observations, this procedure can be applied for large-scale synthesis of Ag-NPs and other notable metal nanoparticles with medicinal and industrial use.